13-17 September 2021 Virtual Conference
Virtual
Europe/Vienna timezone
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Roll-to-roll imprinting of biochips used in multiplexed DNA testing via large-scale manufacturing

16 Sep 2021, 12:10
20m
Room 2

Room 2

Oral Presentation A8. Multi-purpose materials (electronic, magnetic, thermal, sensors/actuators, network materials)- incl. A7 & A10 A8_Multi-purpose materials (electronic, magnetic, thermal, sensors/actuators, network materials)

Speaker

Dr Anja Haase (JOANNEUM RESEARCH Forschungsgesellschaft mbH, Materials-Institute for Surface Technologies and Photonics, A-8160 Weiz, Austria. )

Description

Developing a complete process chain and realization of production lines for bioanalytical lab-on-chip devices. The process is based on high-throughput R2R UV micro/nano- imprinting on a hundred meters-long flexible, polymeric foil in combination with complementary bio-printing and manufacturing technologies. Via our pilot line, R2R UV nanoimprint lithography (NIL) is performed at a foil followed by a gravure coating with a custom-developed photoresin with tuneable properties. [1] Our technology enables producing various kinds of probe DNA/protein functionalised biochip sections on foils; such as, capillary force driven fluidic channels/pumps used for specific bio-detections via in-vitro diagnostic products. Further, production of optical structures with different configurations and geometries for disposable in-vitro chips is possible. Following R2R imprinting of the desired parts, bio-functionalisation via a secondary R2R process line, is achieved using a custom-made, semi-automated micro-array spotting unit. With layout design flexibility and rapid prototyping possibilities, up to 7500 biochips per 100 meter are produced via our technology. For obtaining effectively working and massively produced lab-on-foil products, our production is demonstrated in our recent article [2] with a model application of in-vitro multiplexed DNA testing for markers of a methicillin resistant pathogen. Our foil chips, either parts integrated or completely produced with our R2R UV-NIL pilot line, are complete replacement of the commercially available and disposable chips of that model testing system. In our article, a novel micro- and nano- fabrication technology combined with a DNA micro-spotting line to produced disposable in-vitro diagnostic DNA chips is presented.

REFERENCES
[1] M. Leitgeb et al., ACS Nano, 10, (2016) 4926-4941.
[2] P. Toren et al., Lab on a Chip, 20, (2020) 4106-4117.

ACKNOWLEDGEMENTS
This project has received funding from the European Union’s Horizon 2020 research and innovation programmes under grant agreements no 871345 and 862092.

Speaker Country Austria

Primary authors

Dr Pelin Toren (JOANNEUM RESEARCH Forschungsgesellschaft GmbH, Materials-Institute for Surface Technologies and Photonics) Dr Anja Haase (JOANNEUM RESEARCH Forschungsgesellschaft mbH, Materials-Institute for Surface Technologies and Photonics, A-8160 Weiz, Austria. ) Dr Dieter Nees (JOANNEUM RESEARCH Forschungsgesellschaft mbH, Materials-Institute for Surface Technologies and Photonics, A-8160 Weiz, Austria. ) Stephan Ruttloff (JOANNEUM RESEARCH Forschungsgesellschaft mbH, Materials-Institute for Surface Technologies and Photonics, A-8160 Weiz, Austria. ) Johannes Götz (JOANNEUM RESEARCH Forschungsgesellschaft mbH, Materials-Institute for Surface Technologies and Photonics, A-8160 Weiz, Austria. ) Dr Markus Rumpler (JOANNEUM RESEARCH Forschungsgesellschaft mbH, Health- Institute for Biomedicine and Health Sciences, 8010 Graz, Austria.) Dr Manuel W. Thesen (micro resist technology GmbH, 12555 Berlin, Germany.) Dr Max Sonnleitner (GENSPEED Biotech GmbH, 4261 Rainbach, Austria.) Dr Wilfried Weigel (SCIENION AG, D-12489 Berlin, Germany. ) Dr Martin Smolka (JOANNEUM RESEARCH Forschungsgesellschaft mbH, Materials-Institute for Surface Technologies and Photonics, A-8160 Weiz, Austria. ) Dr Barbara Stadlober (JOANNEUM RESEARCH Forschungsgesellschaft mbH, Materials-Institute for Surface Technologies and Photonics, A-8160 Weiz, Austria. ) Dr Jan Hesse (JOANNEUM RESEARCH Forschungsgesellschaft mbH, Materials-Institute for Surface Technologies and Photonics, A-8160 Weiz, Austria. )

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