Speaker
Description
Developing a complete process chain and realization of production lines for bioanalytical lab-on-chip devices. The process is based on high-throughput R2R UV micro/nano- imprinting on a hundred meters-long flexible, polymeric foil in combination with complementary bio-printing and manufacturing technologies. Via our pilot line, R2R UV nanoimprint lithography (NIL) is performed at a foil followed by a gravure coating with a custom-developed photoresin with tuneable properties. [1] Our technology enables producing various kinds of probe DNA/protein functionalised biochip sections on foils; such as, capillary force driven fluidic channels/pumps used for specific bio-detections via in-vitro diagnostic products. Further, production of optical structures with different configurations and geometries for disposable in-vitro chips is possible. Following R2R imprinting of the desired parts, bio-functionalisation via a secondary R2R process line, is achieved using a custom-made, semi-automated micro-array spotting unit. With layout design flexibility and rapid prototyping possibilities, up to 7500 biochips per 100 meter are produced via our technology. For obtaining effectively working and massively produced lab-on-foil products, our production is demonstrated in our recent article [2] with a model application of in-vitro multiplexed DNA testing for markers of a methicillin resistant pathogen. Our foil chips, either parts integrated or completely produced with our R2R UV-NIL pilot line, are complete replacement of the commercially available and disposable chips of that model testing system. In our article, a novel micro- and nano- fabrication technology combined with a DNA micro-spotting line to produced disposable in-vitro diagnostic DNA chips is presented.
REFERENCES
[1] M. Leitgeb et al., ACS Nano, 10, (2016) 4926-4941.
[2] P. Toren et al., Lab on a Chip, 20, (2020) 4106-4117.
ACKNOWLEDGEMENTS
This project has received funding from the European Union’s Horizon 2020 research and innovation programmes under grant agreements no 871345 and 862092.
| Speaker Country | Austria |
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